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THE™ His Tag Antibody, mAb, Mouse

	價(jià)格：元(人民幣)	產(chǎn)地：本地
	最少起訂量：1	發(fā)貨地：本地至全國(guó)
	上架時(shí)間：2022-07-31 00:41:49	瀏覽量：24
	漠寶（廈門）生物科技有限公司
	經(jīng)營(yíng)模式：其他	公司類型：[db:公司類型]
	所屬行業(yè)：	主要客戶：全國(guó)
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聯(lián)系人:高智星 ()	手機(jī):15860722185
電話:	傳真:
郵箱:2598326770@qq.com	地址:福建省廈門市翔安火炬高新區(qū)翔星路98號(hào)強(qiáng)業(yè)樓406室

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產(chǎn)品簡(jiǎn)介

品牌貨號(hào) 規(guī)格供貨周期應(yīng)用領(lǐng)域

其他品牌	A00186
100ug	一周
生物產(chǎn)業(yè)

THE&trade; His Tag Antibody, mAb, Mouse （subtype IgG1） has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags.

詳情介紹

Description	Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THE™ His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Host Species	Mouse
Conjugation	Unconjugated
Immunogen	A synthetic peptide HHHHHH coupled to KLH
Fusion Partner	Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification	Protein A affinity column

特性

Subclass	IgG1, k
Clone ID	6G2A9
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	THE™ His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note	GenScript can offer this product according to your requirement, including product size, buffer components,etc.

應(yīng)用

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western Blot: 0.1-0.2 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Flow cytometry (FACS): 1 µg/ml
TR-FRET assays: user-optimized
Other applications: user-optimized

漠寶（廈門）生物科技有限公司是一家致力于生命科學(xué)和生物技術(shù)領(lǐng)域的企業(yè)，由廈門大學(xué)研究生創(chuàng)辦。公司主要專注于生命科學(xué)科研試劑、耗材、儀器等科研用品的開發(fā)與銷售。公司主要代理歐美著名生物技術(shù)公司的產(chǎn)品，能為生命科學(xué)多研究領(lǐng)域的科研人員提供全面的產(chǎn)品。我們相信經(jīng)過幾年的努力，漠寶生物一定能成為分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等領(lǐng)域科研產(chǎn)品的供應(yīng)商。
      漠寶生物將以更好的客戶服務(wù)、完備的產(chǎn)品供應(yīng)、深厚的專業(yè)知識(shí)和優(yōu)越的人脈資源，在同行業(yè)中保持良好的企業(yè)形象和競(jìng)爭(zhēng)優(yōu)勢(shì)。我們將不斷引進(jìn)優(yōu)質(zhì)的產(chǎn)品，滿足廣大客戶的需求，在世界各國(guó)大力發(fā)展生命科學(xué)的時(shí)代背景下，我們將以為“中國(guó)生命科學(xué)發(fā)展而盡上一份綿薄之力"為使命，為科學(xué)人員創(chuàng)造的科研成果提供強(qiáng)有力的支撐。
      展望：公司將借助于廈門大學(xué)細(xì)胞應(yīng)激生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室的人才和技術(shù)優(yōu)勢(shì)，致力于研發(fā)、生產(chǎn)出品質(zhì)優(yōu)越的生命科學(xué)研究中用到的試劑、抗體、試劑盒等，力爭(zhēng)在不久的將來將漠寶打造為生命科學(xué)領(lǐng)域的。
      公司部分代理品牌-- Genscript（金斯瑞）、Excell Bio（依科賽）、SAB、Cyagen（賽業(yè)）、SBI、Bioteke（百泰克）、Crystalgen（科晶）、Solarbio（索萊寶）等。

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